Total Oxidant Status (TOS) Assay Kit

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- Package Insert Of TOS Assay Kit.pdf

- RelatedArticles.pdf

 

Summary and Explanation
Reactive oxygen and nitrogen species are produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms that remove them via enzymatic and non-enzymatic antioxidative mechanisms. Under certain conditions, the increase in oxidants and decrease in antioxidants cannot be prevented, and the oxidative or in over 100 disorders, develops.

Serum (or plasma) concentrations of different oxidant species can be measured in laboratories separately, but the measurements are time-consuming, labor-intensive and costly and require complicated techniques2. Since the measurement of different oxidant molecules separately is not practical and their oxidant effects are additive, the total oxidant status (TOS) of a sample is measured and this is named total peroxide (TP)1,3,4,5, serum oxidation activity (SOA)6, reactive oxygen metabolites (ROM)7 or some other synonyms.


Principle of Assay
Oxidants present in the sample oxidize the ferrous ion–chelator complex to ferric ion. The oxidation reaction is prolonged by enhancer molecules, which are abundantly present in the reaction medium. The ferric ion makes a colored complex with chromogen in an acidic medium. The color intensity, which can be measured spectrophotometrically, is related to the total amount of oxidant molecules present in the sample. The assay is calibrated with hydrogen peroxide and the results are expressed in terms of micromolar hydrogen peroxide equivalent per liter (μmol H2O2 Equiv./L)8.


Components
All reagents are ready to use.

  • Reagent (1Assay Buffer) : 50 ml X 1 
  • Reagent 2 (Prochromogen solution) : 10 ml X 1 
  • Standard 1 (Blank Solution)* (Not included) 
  • Standard 2 Solution** : 10 ml X 1

*Use any deionised-water

(0.0 μmol H2O2 Equiv./L) [This solution is used to check kit quality and not used for calibration processes. If the obtained absorbance using this solution is greater than 0.500, discharge this kit please].
**[Stock Stabilized Standard Solution (SSSS)] (800 mM H2O2Equiv./L)


Storage Conditions
This kit should be stored at 2-8oC.


Additional Items Required
A spectrophotometer or a plate reader or an automated biochemistry analyzer.


Samples
Blood serum, heparinised plasma, semen plasma, saliva, urine, cell lysates and tissue homogenates can be used as sample.


 
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