Copper is a very important trace element for human health due to its key roles in biological processes such as energy and iron metabolism, defense against antioxidants, synthesis of neuropeptides, and immune system functions. In case of copper deficiency, significant symptoms such as neutropenia, anemia and abnormalities in bone structure are observed, while hepatocerebral and neurodegenerative findings are seen in toxicity.
Copper found in the samples change the red-orange color of DiBr-PAESA to violet under acidic conditions. The change of absorbance at 572 nm is proportional to total copper concentration in the sample. The assay can be calibrated with copper sulfate dissolved in deionized water.
|Reagent 1||1 x 30 ml|
|Reagent 2||1 x 8 ml|
|Standard||1 x 4 ml|
|Quality Control - Level 1||1 x 4 ml|
|Quality Control - Level 2||1 x 4 ml|
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