Total Antioxidant Status Assay Kit


Reactive oxygen species (ROS) is produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms that remove them via enzymatic and nonenzymatic antioxidative mechanisms. Under certain conditions, the increase in oxidants and decrease in antioxidants cannot be prevented, and the oxidative or in over 100 disorders, develops . Antioxidant molecules prevent or inhibit these harmful reactions. 


Reactive oxygen species (ROS) is produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms that remove them via enzymatic and nonenzymatic antioxidative mechanisms. Under certain conditions, the increase in oxidants and decrease in antioxidants cannot be prevented, and the oxidative or in over 100 disorders, develops .

Antioxidant molecules prevent or inhibit these harmful reactions. Serum (or plasma) concentrations of different antioxidants can be measured in laboratories separately, but the measurements are time-consuming, labor-intensive, costly, and require complicated techniques. Because the measurement measurement of different antioxidant molecules separately is not practical and their antioxidant effects are additive, the total antioxidant capacity of a sample is measured, and this is called total antioxidant capacity (TAC)  , total antioxidant activity (TAA)  , total antioxidant power (TAOP)  , total antioxidant status (TAS)  , total antioxidant response  , or other synonyms.

In the test, antioxidants in the sample reduce dark blue-green colored ABTS radical to colorless reduced ABTS form. The change of absorbance at 660 nm is related with total antioxidant level of the sample. The assay is calibrated with a stable antioxidant standard solution which is traditionally named as Trolox Equivalent that is a vitamin E analog .



Evaluation of oxidant and antioxidant status and relation with prolidase in systemic sclerosis

E Savas, N Aksoy, Y Pehlivan, ZA Sayiner… - Wiener klinische …, 2014 - Springer
… The TOS of serum was determined using a novel-automated measurement method, also
developed by Erel [16], the Rel Assay (Rel Assay Diagnostics kit, Mega Tip, Gaziantep, Turkey) …
TOS total oxidant status, TAS total antioxidant status, OSI oxidative stress index …  Cited by 14 Related articles All 6 versions

[HTML] Association between insulin resistance and oxidative stress parameters in obese adolescents with non-alcoholic fatty liver disease

Ö PirgonH Bilgin, F Çekmez, H Kurku… - Journal of clinical …, 2013 - ncbi.nlm.nih.gov
… Plasma total antioxidant status (TAS) and total oxidant status (TOS) level measurements (REL
Assay Diagnostics) were done in all participants … TAS Determination. TAS was measured
colorimetrically using the Total Antioxidant Status kit (15) (REL Assay Diagnostics) …  Cited by 45 Related articles All 12 versions

Influence of smoking on interleukin‐1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after …

H Toker, A Akpınar, H Aydın… - Journal of periodontal …, 2012 - Wiley Online Library
… TOS and TAS levels were measured using commercially available kits (Rel Assay, Mega Tıp,
Gaziantep, Turkey) by Erel's colorimetric method (21,22) at an … Table 3. Interleukin-1beta (IL-1β)
level, total oxidant status (TOS) and total antioxidant status (TAS) in gingival …  Cited by 40 Related articles All 7 versions

Antiapoptotic and antioxidant effects of GSPE in preventing cyclosporine A-induced cardiotoxicity

G Özkan, S Ulusoy, M Alkanat, A Orem, B Akcan… - Renal failure, 2012 - Taylor & Francis
… Tissue TAS, TOS, and OSI levels were assayed as previously described,11 using commercial
assay kits (Rel Assay Diagnostics, Gaziantep … Notes: CsA, cyclosporine A; GSPE, grape seed
proanthocyanidin extract; TOS, total oxidant system; TAS, total antioxidant system; OSI …  Cited by 24 Related articles All 3 versions

Evaluation of total oxidative stress and total antioxidant status in cows with natural bovine herpesvirus-1 infection

R Durgut, VS Ataseven, A Sağkan-Öztürk… - Journal of animal …, 2013 - academic.oup.com
… E and C increased antioxidant capacity. Pagnini et al. (2004) indicated that antioxidants prevented
oxidative stress; however, they failed to stop virus growth. The objective of this study is to
demonstrate the relationship among total antioxidative status (TAS), total oxidant status …  Cited by 13 Related articles All 8 versions

The protection of selenium on adriamycin-induced mitochondrial damage in rat

E Taskin, N Dursun - Biological trace element research, 2012 - Springer
… Mitochondrial ATP content from each probe was assessed in duplicate. Biochemical Analysis.
Measurement of Total Antioxidant Status. Total antioxidant status (TAS) of the cytosol and
of the kidney cell mitochondria were measured using Rel Assay Kit …  Cited by 26 Related articles All 10 versions

[HTML] Evaluation of total antioxidant status, total oxidant status and oxidative stress index in patients with alopecia areata

S Ozturk, O Ozcan, AB Gurpinar, Y Can… - … journal of clinical and …, 2014 - ncbi.nlm.nih.gov
… from the cells by centrifugation at 3000 rpm for 15 min and were stored at -80°C and used for
the analysis of the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS). Analysis of
blood samples. Serum TAS and TOS were determined with kits (Rel Assay Diagnostics kit …  Cited by 23 Related articles All 10 versions

[PDF] Antioxidant properties of wild edible mushroom Pleurotus eryngii collected from Tunceli province of Turkey.

NC Yildirim, S Turkoglu, N Yildirim… - Digest Journal of …, 2012 - chalcogen.ro
… 2.7 Total Antioxidant Status (TAS) Total antioxidant status was determined by using Rel assay
diagnostics tas assay kit (Lot.RL024) by Multiscan FC (Thermo). Antioxidants in the sample reduce
dark blue-green colored ABTS radical to colorless reduced ABTS form …  Cited by 19 Related articles All 4 versions [PDF] hindawi.com

The association between diabetic retinopathy and levels of ischemia-modified albumin, total thiol, total antioxidant capacity, and total oxidative stress in serum and …

K Kirboga, AV Ozec, M Kosker, A Dursun… - Journal of …, 2014 - hindawi.com
… equiv./L). 2.3.4. Total Antioxidant Status Assay. TAS was determined using commercial
Rel Assay Diagnostic kits (Gaziantep, Turkey) with an autoanalyzer LX20-Pro from
Beckman-Coulter (Woerden, The Netherlands). The method …  Cited by 9 Related articles All 12 versions [PDF] researchgate.net

Effect of Low‐Dose Doxycycline on Serum Oxidative Status, Gingival Antioxidant Levels, and Alveolar Bone Loss in Experimental Periodontitis in Rats

A Yağan, S Kesim, N Liman - Journal of periodontology, 2014 - Wiley Online Library
… Fully Automated 3rd Generation Total Antioxidant Status (TAS) Assay Kit, Product Code RL0017,
Rel Assay Diagnostics, Mega Tıp, Gaziantep, Turkey. ## Fully Automated Total Oxidant Status
(TOS) Assay Kit, Product Code RL0024, Rel Assay Diagnostics, Mega Tıp …  Cited by 36 Related articles All 8 versions

Reagent 1 - Buffer1 x 30 ml
Reagent 2 - ABTS Radical Cation1 x 8 ml
Standard1 x 4 ml
Quality Control - Level 11 x 4 ml
Quality Control - Level 21 x 4 ml

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2. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176–86.

3. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004 Apr;37(4):277-85.

4. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54(5):356 – 61.

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6. Aycicek A, Iscan A, Erel O, Akcali M, Selek S. Total antioxidant/oxidant status in meningism and meningitis. Pediatr Neurol. 2006 Dec;35(6):382-6.

7.Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004 Feb;37(2):112-9.