Paraoxonase Assay Kit


Paraoxonase-1 (PON1) s an hgh densty lpoproten (HDL)-assocated enzyme wth antoxdant and antatherogenc functons, protectng lpoprotens aganst oxdatve modficaton  It also catalyzes the hydrolyss of organophosphates such as paraoxon and aromatc carboxylc acd esters of fatty acds. It has been shown that serum paraoxonase actvty decrease n dabetes melltus, coronary artery dsease, hypercholesterolaema, ron deficency annema, heepatts, crrhoss, prostate cancer, tuberculoss and nfla mattons. 

Paraoxonase-1 (PON1) s an hgh densty lpoproten (HDL)-assocated enzyme wth antoxdant and antatherogenc functons, protectng lpoprotens aganst oxdatve modficaton  It also catalyzes the hydrolyss of organophosphates such as paraoxon and aromatc carboxylc acd esters of fatty acds. It has been shown that serum paraoxonase actvty decrease n dabetes melltus, coronary artery dsease, hypercholesterolaema, ron deficency annema, heepatts, crrhoss, prostate cancer, tuberculoss and nfla mattons. 

Fully automated paraoxonase actvty measurement method conssts of two dfferent seequenta reagents. The first reagent s an approprate Trs buffer and  t also contans calcum on, whch s a cofactor of PON1 enzyme. The second reagent s a new developed stable substrate soluton. The sample s mxed wth the Reagent 1 and the substrate soluton s added. Lnear ncrease of the absorbance of p-ntrophenol, produced from paraoxon, s followed at knetc measurement mode. Nonenzymatc hydrolyss of paraoxon was substracted from the total rate of hydrolyss. The molar absorptvty of p-ntrophenol s 18,290 M-1 cm-1 and one unt of paraoxonase actvty s equal to 1 mol of paraoxon hydrolyzed per lter per mnute at 37oC 

 

Serum paraoxonase activity and oxidative status in subjects with alopecia areata

SG BilgiliH OzkolAS KaradagHU Ozkol… - … and ocular toxicology, 2013 - Taylor & Francis
… Measurement of serum paraoxonase activity The PON1 activity was measured by reading
the absorbances with a spectrophotometer (Genesys 10 UV Scanning UV/VIS
Spectrophotometer; Shimadzu) using kits (Rel Assay Diagnostics kit; Mega T|p)25 …  Cited by 17 Related articles All 3 versions

[HTML] The effect of date seed (Phoenix dactylifera) extract on paraoxonase and arylesterase activities in hypercholesterolemic rats

MR Takaeidi, A JahangiriMJ Khodayar… - … journal of natural …, 2014 - ncbi.nlm.nih.gov
… Paraoxonase, arylesterase and antioxidant activity were measured using commercially available
kits (Rel Assay Diagnostics®, MEGA TIP San, Turkey) … Paraoxonase (PON 1) as a biomarker
of susceptibility for organophosphate toxicity. Biomarkers. 2003;8(1):1–12 …  Cited by 17 Related articles All 11 versions

Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters

C Ertürk, MA Altay, Ş Selek… - Scandinavian journal of …, 2012 - Taylor & Francis
… TAC of serum was determined using an automated measurement method (Rel ® assay diagnostic
kits) of Erel [29], in which the hydroxyl radical, the … M, Rosenblat M, Billecke S, Erogul J, Sorenson
R, [13] Bisgaier CL, Newton RS, La Du B. Human serum paraoxonase (PON 1) is …  Cited by 9 Related articles All 4 versions

Serum paraoxonase 1 activity and oxidative stress in pediatric patients with pulmonary tuberculosis

E Torun, AH Gedik, E Cakir, T Umutoglu… - Medical Principles and …, 2014 - karger.com
… The PON1 enzyme activity was measured by using commercially available kits (Rel Assay
Diagnostics) … The molar absorptivity of p-nitrophenol is 18,290 M -1 cm -1 and 1 unit of
paraoxonase activity is equal to 1 mol of paraoxon hydrolyzed per liter per minute at 37°C [16] …  Cited by 11 Related articles All 8 versions

Serum paraoxonase activity and oxidative stress and their relationship with obesity-related metabolic syndrome and non-alcoholic fatty liver disease in obese children …

E Torun, S Gökçe, S Aydın, Y Cesur - Journal of Pediatric …, 2014 - degruyter.com
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(0). (0) My CartAdded To Cart. Check Out. Menu: Subjects …  Cited by 19 Related articles All 5 versions

Evaluation of oxidative stress, the activities of paraoxonase and arylesterase in patients with subclinic hypothyroidism

E Cebeci, FA Oner, M Usta - Acta Bio Medica Atenei …, 2012 - mattioli1885journals.com
… activity of the serum samples was exam- ined using full-automatized method developed by Rel
Assay Diagnostics® (Mega … in medium without NaCl (basal paraoxonase ac- tivity) and with NaCl
(salt-stimulated paraoxonase ac- tivity) … Measurement of PON 1 Arylesterase Activity …  Cited by 12 Related articles All 4 versions [HTML] tandfonline.com

Serum paraoxonase-1 enzyme activities and oxidative stress levels in patients with esophageal squamous cell carcinoma

A Sehitogulları, M Aslan, F Sayır, A Kahraman… - Redox …, 2014 - Taylor & Francis
… of paraoxonase and arylesterase activities Paraoxonase and arylesterase activities were
measured using commercially available kits (Rel Assay, Gaziantep, Turkey) … Human serum
paraoxonase (PON 1) is inac- tivated by oxidized low density lipoprotein and preserved by anti …  Cited by 12 Related articles All 3 versions

[PDF] Oxidized low-density protein, interleukin-6 and tumor necrosis factor alpha levels and paraoxonase activities in hypertensive patients

A Gönenç - Turk J Pharm Sci, 2012 - eski.teb.org.tr
… Determination of paraoxonase activities Serum paraoxonase activity were measured using
commercially available kit (Rel Assay Diagnostics, Gaziantep, Turkey). Fully automated
paraoxonase activity measurement method consists of two different sequential reagents …  Cited by 7 Related articles All 5 versions Serum paraoxonase‐1 activities and oxidative status in patients with plaque‐type psoriasis with/without metabolic syndrome
M Usta, E Turan, H Aral, BB Inal… - Journal of clinical …, 2011 - Wiley Online Library
… Measurements of Serum Paraoxonase and Arylesterase Activities of PON1 Enzyme. Serum
paraoxonase and arylesterase activities were determined using commercially available
kits (Rel Assay Diagnostics ® , Mega Tıp, Gaziantep, Turkey) …  Cited by 19 Related articles All 5 versions


Reagent 1 - Buffer1 x 30 ml
Reagent 2 - Substrate1 x 8 ml


1. Suchocka Z, Swatowska J, Pachecka J, Suchocka P; RP-HPLC determination of Paraoxonase activity in human blood serum; J of Pharmaceutical and Biomedical Analysis  2006;  42:113-119

2. Marchegiani F, Marra M, Olivieri F, Cardelli M, James RW, Boemi M, Franceschi C.   Paraoxonase 1: genetics and activities during aging. Rejuvenation Res. 2008 Mar;11(1):113-27.

3. Hofer  Hofer SE, Bennetts B, Chan AK, Holloway B, Karschimkus C, Jenkins AJ, Silink M, Donaghue KC. Association between PON 1 polymorphisms, PON activity and diabetes complications. J Diabetes Complications. 2006 20(5):322 -8.

4. Mackness MI, Harty D, Bhatnagar D, Winocour PH, Arrol S, Ishola M, Durrington PN. Serum paraoxonase activity in familial hypercholesterolaemia and insulin-dependent diabetes mellitus. Atherosclerosis. 1991; 86(2-3):193-9.

5. Horoz M, Aslan M, Selek S, Koylu AO, Bolukbas C, Bolukbas FF, Celik H, Erel O.   PON1 status in haemodialysis patients and the impact of hepatitis C infection. Clin Biochem. 2007;40(9-10):609-14.

6. Gur M, Aslan M, Yildiz A, Demirbag R, Yilmaz R, Selek S, Erel O, Ozdogru I.  Paraoxonase and arylesterase activities in coronary artery disease. Eur J Clin Invest. 2006 Nov;36(11):779-87.

7. Aslan M, Kosecik M, Horoz M, Selek S, Celik H, Erel O. Assessment of paraoxonase and arylesterase activities in patients with iron deficiency anemia. Atherosclerosis. 2007 Apr;191(2):397-402.

8. Aslan M, Nazligul Y, Horoz M, Bolukbas C, Bolukbas FF, Gur M, Celik H, Erel O.  Serum paraoxonase-1 activity in Helicobacter pylori infected subjects. Atherosclerosis. 2008 Jan;19 6(1):270-4.