Total Oxidant Status Assay Kit

Reactive oxygen species (ROS) is produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms that remove them via enzymatic and nonenzymatic antioxidative mechanisms. Under certain conditions, the increase in oxidants and decrease in antioxidants cannot be prevented, and the oxidative or in over 100 disorders, develops .

Reactive oxygen and nitrogen species are produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms that remove them via enzymatic and non-enzymatic antioxidative mechanisms. Under certain conditions, the increase in oxidants and decrease in antioxidants cannot be prevented, and the oxidative or in over 100 disorders, develops. Serum (or plasma) concentrations of different oxidant species can be measured in laboratories separately, but the measurements are time-consuming, labor-intensive and costly and require complicated techniques. Since the measurement of different oxidant molecules separately is not practical and their oxidant effects are additive, the total oxidant status (TOS) of a sample is measured and this is named total peroxide (TP) , serum oxidation activity (SOA) , reactive oxygen metabolites (ROM) or some other synonyms.

In the test, oxidants present in the sample oxidize the ferrous ion– chelator complex to ferric ion. The oxidation reaction is prolonged by enhancer molecules, which are abundantly present in the reaction medium. The ferric ion makes a colored complex with chromogen in an acidic medium. The color intensity, which can be measured spectrophotometrically, is related to the total amount of oxidant molecules present in the sample. The assay is calibrated with hydrogen peroxide and the results are expressed in terms of micromolar hydrogen peroxide equivalent per liter (μmol H2O2 Equiv./L) .

[PDF] Serum paraoxonase, arylesterase activity and oxidative status in patients with obstructive sleep apnea syndrome (OSAS)

E Baysal, S Taysi, N Aksoy, M Uyar… - Eur Rev Med …, 2012 -
… The data were expressed as mmol Trolox equivalents/L. Measurement of the Total Oxidant Status
TOS measurement was performed using the Erel method15. The reagent was manufactured
by Rel Assay (Rel Assay®, Diagnostics kits, Mega Tıp, Gaziantep, Turkey) …  Cited by 26 Related articles All 5 versions Evaluation of oxidant and antioxidant status and relation with prolidase in systemic sclerosis
E Savas, N Aksoy, Y Pehlivan, ZA Sayiner… - Wiener klinische …, 2014 - Springer
… The TOS of serum was determined using a novel-automated measurement method, also
developed by Erel [16], the Rel Assay (Rel Assay Diagnostics kit, Mega … which can be measured
spectrophotometrically, is related to the quantity of oxidant molecules present …  Cited by 14 Related articles All 6 versions

[HTML] Association between insulin resistance and oxidative stress parameters in obese adolescents with non-alcoholic fatty liver disease

Ö PirgonH Bilgin, F Çekmez, H Kurku… - Journal of clinical …, 2013 -
… Plasma total antioxidant status (TAS) and total oxidant status (TOS) level measurements
(REL Assay Diagnostics) were done in all participants. The ratio of TOS to TAS was regarded
as an oxidative stress index (OSI), an indicator of the degree of OS …  Cited by 45 Related articles All 12 versions

Influence of smoking on interleukin‐1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after …

H Toker, A Akpınar, H Aydın… - Journal of periodontal …, 2012 - Wiley Online Library
… TOS and TAS levels were measured using commercially available kits (Rel Assay, Mega Tıp,
Gaziantep, Turkey) by Erel's colorimetric method (21,22) at an absorbance of 520 nm, as
previously … Table 3. Interleukin-1beta (IL-1β) level, total oxidant status (TOS) and total …  Cited by 40 Related articles All 7 versions

The protection of selenium on adriamycin-induced mitochondrial damage in rat

E Taskin, N Dursun - Biological trace element research, 2012 - Springer
… The results were expressed in millimoles Trolox equivalent per liter. Measurement
of Total Oxidant Status. The total oxidant status (TOS) of the cytosol and of the kidney
cell mitochondria were measured using Rel Assay Kit. The …  Cited by 26 Related articles All 10 versions

Oxidative stress in chronic otitis media

E Baysal, N Aksoy, F Kara, S Taysi, A Taşkın… - European Archives of …, 2013 - Springer
… The measurement of TOS was performed using the Erel method, in which oxidants present in
the … The Rel Assay (Rel Assay ® Diagnostics kits, Mega Tıp, Gaziantep, Turkey) was used [14 … The
color intensity is related to the total amount of oxidant molecules present in the sample …  Cited by 19 Related articles All 7 versions

The relationship between oxidative stress and autoimmunity in Hashimoto's thyroiditis

I Ates, FM Yilmaz, M Altay, N Yilmaz, D Berker… - European journal of …, 2015 - EFES
… levels were measured with the colorimetric method using a commercial kit (Rel Assay Diagnostics,
REF … and anti-TPO, while a positive correlation was present between TOS and anti … found a positive
correlation of the oxidant molecules malondialdehyde and protein carbonyl with …  Cited by 17 Related articles All 5 versions

[PDF] Nitric oxide levels, total antioxidant and oxidant capacity in cattle with foot-and-mouth-Disease

K Bozukluhan, E Atakisi, O Atakisi - Kafkas Univ Vet Fak Derg, 2013 -
… Serum total antioxidant and oxidant capacities were mea- sured colorimetrically
(PowerWave XS, BioTek, Instruments, USA) using a commercial kit (Rel Assay
Diagnostic, Turkey). Trolox and hydrogen peroxide were used as …  Cited by 19 Related articles All 10 versions Anti‐apoptotic and anti‐oxidant effects of grape seed proanthocyanidin extract in preventing cyclosporine A‐induced nephropathy
S Ulusoy, G Ozkan, FB Yucesan, Ş Ersöz, A Orem… - …, 2012 - Wiley Online Library
… Tissue TAS, TOS, and OSI levels were assayed as described previously 17 using commercial
assay kits (Rel Assay Diagnostics, Gaziantep, Turkey, product numbers RL0017 … Effect of grape
seed proanthocyanidin extract (GSPE) on total oxidant system (TOS), total anti …  Cited by 37 Related articles All 5 versions

Relationship between oxidative stress parameters and asymptomatic organ damage in hypertensive patients without diabetes mellitus

I Ates, N Ozkayar, C Topcuoglu… - Scandinavian …, 2015 - Taylor & Francis
… Serum arylesterase level was measured with a com- mercial kit (Rel Assay Diagnostics, Turkey,
REF No: RL0055, LOT No … organ damage; LVMI, Left ventricular mass index; CIMT, Carotid intima –
media thickness; TAS, Total antioxidant status; TOS, Total oxidant status; OSI …  Cited by 12 Related articles All 3 versions

Reagent 1 - Buffer1 x 30 ml
Reagent 2 - Prochromogen1 x 8 ml
Standard1 x 4 ml
Quality Control - Level 11 x 4 ml
Quality Control - Level 21 x 4 ml

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